• 介紹：

  羅丹明123 (Rhodamine 123、羅丹明123, Rh 123)是一種常用的陽離子綠色熒光染料，可以透過細(xì)胞膜從而在活細(xì)胞線粒體內(nèi)聚集，并發(fā)出黃綠色熒光。廣泛用作檢測線粒體膜電位的熒光探針，并且對細(xì)胞沒有任何毒性。 

  溶液的配制

  用DMSO溶解，終濃度為1-5 mM。溶液分裝，避光保存于-20 ºC。

  羅丹明123使用方法

  1. 用載玻片準(zhǔn)備細(xì)胞。細(xì)胞數(shù)目應(yīng)為 5×104～5×105個/mL。

  2. 孵育該載玻片，用 PBS或Hank’s 液洗滌細(xì)胞。

  3. 用培養(yǎng)基稀釋 Rh123母液以制備 1～20µM Rh123緩沖液。具體工作濃度取決于細(xì)胞類型和細(xì)胞濃度。

  4. 將 Rh123緩沖液加入載玻片并在37℃下孵育 30 分鐘至 1 小時。

  5. 去除 Rh123 緩沖液并用培養(yǎng)基洗滌細(xì)胞（洗滌細(xì)胞后為了固定，加入 10%福爾馬林緩沖液并孵育 15～20 分鐘，接著用 PBS洗滌）。

  6. 用帶有熒光素濾光片的熒光顯微鏡觀察細(xì)胞。

  為了您的安全和健康，請穿實(shí)驗服并戴一次性手套操作。

• 熔點(diǎn)： 235℃

• 溶解性： Soluble  in  ethanol  (20  mg/ml),  DMSO,  methanol,  and  water  (partly  miscible).

• 儲存條件： RT

• 注意：部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

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輸入產(chǎn)品批號：

本計算器可幫助您計算出特定溶液中溶質(zhì)的質(zhì)量、溶液濃度和體積之間的關(guān)系，公式為：

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

• pg ng μg mg g kg =
  fM pM nM μM mM M *
  nL μL mL L *